Advanced Bioimaging Laboratory
Donald Danforth Plant Science Center • St. Louis, MO
Contact Info:
Director Kirk Czymmek, PhD
Pricing:
Available Upon Request
Summary:
The Advanced Bioimaging Laboratory is a 1,500 sq/ft suite of powerful imaging and analysis platforms as well as supporting preparation equipment for routine and advanced plant microscopy applications, including confocal, super-resolution, macro microscopy, wide-field fluorescence, dissecting,and transmission electron microscopy. The facility has the following capabilities:
1. Generate high-resolution 3D and 4D image data
2. Produce large area maps and sophisticated multi-location and analysis routines
3. Perform brightfield, phase contrast, DIC, sensitive imaging of a variety of fluorphores including time-lapse fluorescence imaging
4. Screen for fluorescent protein expression and white light imaging of plant morphology
5. 3D visualization and analysis with deconvolution optical sectioning
The facility also includes equipment for cryo-preparation, processing frozen samples, fast-freeze substitution, ultrathin sectioning, serial sectioning, microdissection, handling frozen sections to glass slides, and sample preparation.
Equipment:
- 405nm (50mW), 488nm (500mW), 561nm (500mW) and 640nm (500mW) diode laser lines
- Ambient tomography holder
- Ceta 16M 4k x 4k CMOS camera
- Confocal data storage on 4 drive RAID 5 configured Buffalo drive with 16 Terabyte of storage capacity
- DelsciMachine micromanipulator
- Diatome Jumbo Histology diamond knife
- eica EM AFS2-FSP automated freeze substitution unit with UV polymerization at cryo-temperatures for sub sequent immunogold labelling
- EMBL grid plunge-freezer
- EMS Freeze Substitution Kit with Shaker for fast-freeze
- Gatan ELSA 698.ULP cryo holder (+/1 70 degrees)
- Leica EM ICE high-pressure freezer
- Leica SP8-X confocal microscope
- 405nm diode laser
- Argon gas laser
- Fluorescence detectors (5), including 3 high quantum efficiency HYD detectors
- Full range of high-resolution optics on an inverted microscope platform
- Resonant scanner
- White light laser (200 laser lines)
- 405nm diode laser
- Leica Ultracut UCT
- MAPS 3 software installed
- Nikon fluorescence dissecting microscope
- Digital camera (Q Imaging)
- Huygen’s deconvolution software package
- Oxford Instruments Imaris Software
- Digital camera (Q Imaging)
- Nikon Ni-E wide-field fluorescence imager with high-resolution objectives
- 16 bit b/w digital camera (Hamamatsu Orca Flash 4.0)
- Color CMOS camera (Nikon Fi-3)
- Z-stepper motor for 3D imaging
- 16 bit b/w digital camera (Hamamatsu Orca Flash 4.0)
- ORS Dragonfly, Zeiss Intellesis, and FIJI Weka Segmentation for machine/deep learning-based image segmentation
- Stereology Analyzer v4.3.3
- Super-resolution data storage on 8 disk bay Synology Disk Station with 72TB in a RAID 6 configuration
- Thermo Scientific Talos L120C G2
- Tousimis critical point dryer and sputter coater
- Vibratome 1000 vibrating microtome
- Zeiss AxioZoom macro microscope with Apotome 2 with high-resolution multiscale fluorescence, confocal, transmitted and reflected slide images, multi-well plates, and intact plant specimens
- Zeiss Elyra7 super-resolution microscope with TIRF, Lattice SIM2, Apotome SIM2, and 2D and 3D single molecule localization microscopy super-resolution capabilities
- Zeiss PALM laser catapulting system with Leica CM1950 and CryoJane system
- ZEN Connect correlative microscopy workspace