ST. LOUIS, MO, January 28, 2019 – The “hidden hunger” caused by micronutrient deficiency is a global threat to human health, with particularly severe impacts in Africa. In Nigeria, 75 percent of preschool children and 67 percent of pregnant women are anemic, and 20 percent of children below five years suffer from zinc deficiency. Iron deficiency anemia affects the immune system, stunts growth and impairs cognitive development in children, while deficiency in zinc causes increased risk of death from diarrhea, stunting and reduced cognitive development. Developing new varieties of a staple food crop with elevated levels of these two minerals could significantly improve diets and health.

In a paper published today in the journal Nature Biotechnology, “Biofortification of field-grown cassava by engineering expression of an iron transporter and ferritin,” Donald Danforth Plant Science Center scientists, led by Narayanan Narayanan, Ph.D., research scientist, and Nigel Taylor, Ph.D., associate member and Dorothy J. King Distinguished Investigator, and an international team of researchers demonstrated that field-grown cassava plants overexpressing a combination of plant genes can accumulate significantly higher concentrations of both iron and zinc. The elevated mineral levels of the ‘biofortified’ cassava storage roots are retained after processing into common foodstuffs and are nutritionally available at levels that could have a significant impact on the health of cassava-consuming populations in West Africa.

“This work showed us that it is possible to raise the iron and zinc content of cassava roots while maintaining yield and other plant characteristics that are important to farmers and consumers,” Taylor said. “We also confirmed that the higher mineral levels don’t disappear during cooking, which means that better nutrition can actually reach the dinner plate and the digestive tract.”

The research reported today took place over a ten-year period and involved more than a dozen scientists working in the laboratory, greenhouse and field locations. Cereal crops with higher mineral levels have been developed using the tools of genetic modification, but non-grass plants such as cassava take up minerals from the soil in a different way.

Ultimately it was a combination of two genes, IRT1 and FER1, from the model plant species Arabidopisis that led to cassava plants with iron concentration levels 6-12 times higher than conventional cassava and zinc concentrations that were 3-10 times higher. “It was a real challenge to find a combination of genes that would raise both iron and zinc levels and maintain them under field conditions without impacting yields,” stated the paper’s lead author, Dr. Narayanan Narayanan.

To identify the impact of food processing on mineral levels in the biofortified cassava, researchers prepared gari and fufu, two common West African foods, by chopping, soaking, fermenting, pressing and roasting cassava. They found that high levels of iron and zinc were retained through these cooking processes and remained available for absorption in the gut following digestion. Ultimately the biofortified cassava could provide 40-50 percent of Estimated Average Requirements (EAR) for iron and 60-70 percent of EAR for zinc for children and women in West Africa.

"Biofortified cassava has the potential to improve the nutrition and health of millions of people in West Africa," said Taylor.  “The higher iron and zinc would be present in every root harvested from these special plants, and therefore in every bite of food prepared from them.”

Researchers in the VIRCA Plus project (cassavaplus.org) are now integrating the high iron and zinc trait into cassava varieties that are popular in Nigeria, with more field evaluations and assessments planned for 2019.  Further development, testing and regulatory review are needed before iron and zinc biofortified cassava could be made available to farmers and consumers in the coming years.

Co-lead authors on the paper are Getu Beyene, Raj Deepika Chauhan, Eliana Gaitán-Solís, Jackson Gehan, Paula Butts, and Paul Anderson, all of the Donald Danforth Plant Science Center; Dimuth Siritunga of the University of Puerto Rico Mayagüez; Ihuoma Okwuonu of the National Root Crops Research Institute, in Umudike, Nigeria; Arthur Wolf from the Cornell High Energy Synchrotron Source, Cornell University; Dulce M. Jiménez-Aguilar from USDA-ARS Children’s Nutrition Research Center, Baylor College of Medicine; Erick Boy from Harvest Plus/International Food Policy Research Institute, and Michael A. Grusak of the USDA-ARS Red River Valley Agricultural Research Center in Fargo, ND.

The research was funded by the Bill & Melinda Gates Foundation through the Global Challenges for Global Health Program (Grant no: OPPGD1484), and US Department of Agriculture, Agricultural Research Service, Cooperative Agreement Number 58-6250-0-008 (to M.A.G.). CHESS is supported by the NSF award DMR-1332208.

About the Donald Danforth Plant Science Center

Founded in 1998, the Donald Danforth Plant Science Center is a not-for-profit research institute with a mission to improve the human condition through plant science. Research, education and outreach aim to have impact at the nexus of food security and the environment, and position the St. Louis region as a world center for plant science. The Center’s work is funded through competitive grants from many sources, including the National Institutes of Health, U.S. Department of Energy, National Science Foundation, and the Bill & Melinda Gates Foundation. Follow us on Twitter at @DanforthCenter.